Cosmetic Nano LPD´s Definition

They are extremly small vesicles

(lower than 300nm)

Mainly made of phospholipids

The phospholipids are organised in bilayers.

Composition

They are mainly made of natural origin phospholipids

Their characteristics are :To have a hydrophilic part ( polar head)

and to have a lipophilic part (apolar tail)

 

Nano LPD´s Phospholipids in bilayer.

Micela Phospholipids in monolayer.

Composition

They are mainly made of natural origin
phospholipids
 Their characteristics are :
To have a hydrophilic part ( polar head)
and to have a lipophilic part (apolar tail)

Phospholipid Structure

Clasification

Size:

• Small (diameter < 100 nm)

•  Big (diameter > 100 nm)
  

•  Number of bilayers:
  

•  Unilamelars
  

•  Oligolamelars
  

•  Multilamelars

Usages and Advantages

They are natural delivery systems of activeing redients
 -They are controlled and released carrier systems
 -They are structure analogues of the cellular membranes (phospholipids)
 -They increase the efficacy and decrease the unwanted side effects of the active ingredients (toxicity)

Analogy between the cellular membrane structure and Nano LPD´s

Usage and Advantages

Image of the Nano LPD´s going through the SC

Study carried out by Dr. A De La Maza. Departament df Surfactants.
CSIC, Barcelona

Damaged Skin. Low lipid content SC

Skin treated with Nano LPD´s. Re-epitheliated SC

Usage and Advantages

The active ingredients reallocate at the interface of the Nano-LPD´s
- Because of the structure and also the phospholipid bilayer composition, the Nano-LPD´s can incorporate: :
- Hydrophilic Actives (within the vesicle)
- Lipophilic Actives (between the layers)

Interaction Nano LPD´s – active ingredient

 

Usages and Advantages

Prolongation of the bioavailability of the active ingredient
- Better absortion, penetration and diffusion of the active ingredient
- Stabilization of the active ingredient
- Introduction of alternative administration ways of the active ingredient

Improvement of the analgesic action of the lidocaine (topically applied)

 

Nano LPD´s Multivitamin

composition and actions

VITAMINS AND THEIR DERIVATIVES IN SKIN CARE

VITAMIN A : Normalize keratinization,Downregulate sebum production in acne,Reverse and treat photodamage,Striae,Cellulite

VITAMIN C : Antioxidant,Regulates collagen synthesis,Formation of stratum-corneum barrier lipids,Regenerates Vitamin E,Provides photoprotection

VITAMIN E : Membrane antioxidant,Protects against VITAMIN E oxidative damage,Provides photoprotection,

VITAMIN F : Cellular regeneration of the membranes and tissues

Multivitamin vitamin A

 

 Multivitamin vitamin C

 

 Multivitamin vitamin E

 

Multivitamin vitamin F

The polyunsatured fatty acids in vitamin F can not be synthesised by the body
• Intervene in cellular regeneration of the membranes and tissues
• Ability to modify states of the skin as dryness, rashes and peeling
• Envigorating properties
• Improve the look of the cutis (also applied to eliminate small folds and wrinkles)

Mixture of polyunsatured fatty acids :
• Linoleic Acid C18:2 50 - 57%
• Linolenic Acid C18:3 0,7 - 1,2%
•Arachidonic Acid C20:4 0,2 - 0,4%

Multivitamin efficacy test

In vitro test :
 -Fibroblast outgrowth
 -MMP inhibition
 -Collagen biosynthesis
 In vivo test :
 -Macro-relief of human skin

 Multivitamin fibroblast outgrowth

 • Objective : evaluate the capability of Nano LPD´s Multivitamin to increase fibroblast growth potential
• Methodology :
• Freshly obtained skin samples from persons of varying age were cut into small pieces and placed in
culture medium to allow outgrowth of fibroblasts
• Four age groups : 18-29, 30-45, 46-60, < 60 years old
• Data are presented as the % of skin pieces from which fibroblast were isolated
• Results :
• Fibroblast growth potential is reduced with increased age
• Fibroblast growth potential is increased with Nano LPD´s Multivitamin treatment

 Multivitamin MMP assays

• Objective : evaluate the capability of Nano LPD´s Multivitamin to inhibit MMP concentration
• Methodology :
• Freshly obtained skin samples from persons of varying age
• Collagenase (MMP-1) levels were measured by hydrolysis and quantitated with Western blot method
• Gelatinase levels (MMP-2 and MMP-9) were measured by gelatin zymography and quantitated by scanning laser densitometry
• Results :
• MMP levels are increased in skin with increasing age
•MMP levels are reduced in skin with Nano LPD´s Multivitamin treatment

• Objective : evaluate the capability of Nano LPD´s Multivitamin to inhibit MMP concentration
• Methodology :
• Freshly obtained skin samples from persons of varying age
• Collagenase (MMP-1) levels were measured by hydrolysis and quantitated with Western blot method
• Gelatinase levels (MMP-2 and MMP-9) were measured by gelatin zymography and quantitated by scanning laser densitometry
• Results :
• MMP levels are increased in skin with increasing age
•MMP levels are reduced in skin with Nano LPD´s Multivitamin treatment

Multivitamin Collagen biosynthesis

• Objective : evaluate the capability of Nano LPD´s Multivitamin to increases collagen synthesis
• Methodology :
• Freshly obtained skin samples from aged persons were incubated for 24h. in keratinocyte basal medium
• Total collagen biosynthesis was assessed by incorporation of [14C] proline into pepsin-resistant, TCA-precipitatable material
• Type I and III procollagen (α1 chain) protein levels were assessed by western blot analysis inmunohistology
• Results :
• Nano LPD´s Multivitamin increases collagen synthesis in aged skin

Macro-relief of human skin

• Objective : evaluate the capability of Nano LPD´s Multivitamin at 5% to reduce wrinkles
• Methodology :
• Determination of the macro-relief of silicon replica from 15 volunteers obteined from the area surround the eyes.
• 4 weeks treatment, analysis of samples at time 0, before the beginning of treatment and at 4 weeks (28 days of application)
• The rugosity average has been assesed through confocal profilometry with a Profilemeter Plμ and stereoscopic microscopy Optech ST3
• Results :
• Reduction of wrinkles of 25%

Multivitamin

New technology :
 -Nanosystems
 -Natural
 -Controlled and sustained release ∅ < 250nm Phospholipid

 Natural active ingredients of proven efficacy
 -Vitamin A
 -Vitamin C
 -Vitamin E
 -Vitamin F

COSMETIC APPLICATIONS :
 Antiageing agent
 Antioxidant
 Prevention of photodamage
 DOSAGE :
 3-5 % OF Nano LPD´s Multivitamin

 

Credit by infinitec activossl.
Photo by infinitec activossl.